用C组链球菌免疫后诱导产生分子均一性的兔抗体。
Induction of rabbit antibody with molecular uniformity after immunization with group C streptococci.
作者信息
Eichmann K, Lackland H, Hood L, Krause R M
出版信息
J Exp Med. 1970 Jan 1;131(1):207-21. doi: 10.1084/jem.131.1.207.
Abstract
Antibodies with uniform properties may occur in rabbits after immunization with Group C streptococci. These precipitating antibodies possess specificity for the group-specific carbohydrate. Not uncommonly, their concentration is between 20 and 40 mg/ml of antiserum. Evidence for molecular uniformity in the case of one of these antibodies, described in detail here, includes: individual antigenic specificity; monodisperse distribution of the light chains by alkaline urea polyacrylamide disc electrophoresis; and a single amino acid in each of the first three N-terminal positions of the light chains. When the amino acid sequence of rabbit antibody b+ light chains (kappa type) are aligned against their human kappa counterparts, a definite homology is observed between the N-terminus of the human and the rabbit variable region.
摘要
用C组链球菌免疫兔子后，可能会产生具有均匀特性的抗体。这些沉淀抗体对群特异性碳水化合物具有特异性。它们的浓度通常在每毫升抗血清20至40毫克之间。这里详细描述的其中一种抗体的分子均匀性证据包括：个体抗原特异性；碱性尿素聚丙烯酰胺圆盘电泳显示轻链呈单分散分布；轻链前三个N端位置的每一个都只有一种氨基酸。当将兔抗体b +轻链（κ型）的氨基酸序列与人κ对应物进行比对时，在人和兔可变区的N端之间观察到明确的同源性。
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Individual Antigenic Specificity of Isolated Antibodies.分离抗体的个体抗原特异性
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety.链球菌细胞壁化学结构的研究。II. C群细胞壁的组成及碳水化合物部分血清学特异性的化学基础。
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