Mozell M M
J Gen Physiol. 1966 Sep;50(1):25-41. doi: 10.1085/jgp.50.1.25.
Activity in two separate regions of the frog olfactory mucosa was sampled by simultaneously recording the summated neural discharges from the olfactory nerve branches originating from them. The difference in the activity from these two regions in response to a stimulus was measured by: (a) the ratio of the response amplitude recorded from the lateral nerve branch to that recorded from the medial nerve branch (LB/MB ratio), (b) the latency difference (or time interval) between these two responses. Equal concentrations of four different odorants were drawn into the nose by an artificially produced sniff of known dimensions. At each concentration in every animal the four chemicals were ranked in order of the magnitudes of their LB/MB ratios and again in order of their latency differences. Regardless of their concentration, the same chemicals fell into the same ranks in different animals. In addition, for each chemical the magnitudes of the ratios and latency differences showed only minimal changes with concentration. Thus, spatiotemporal patterns of relative response magnitudes and latency differences across the mucosa differentially represented the odorants. Such a spatiotemporal code, together with physicochemical considerations, suggested that the nose separates vapors in a manner similar to a gas chromatograph. This is further supported by the previously observed reversal of the ratio patterns with reversal of air flow direction through the olfactory sac.
通过同时记录源自青蛙嗅觉黏膜两个不同区域的嗅神经分支的总和神经放电，对这两个区域的活动进行采样。通过以下方式测量这两个区域对刺激的活动差异：(a) 从外侧神经分支记录的反应幅度与从内侧神经分支记录的反应幅度之比（LB/MB 比），(b) 这两个反应之间的潜伏期差异（或时间间隔）。通过人工产生的具有已知尺寸的吸气，将四种不同气味剂的等浓度溶液吸入鼻腔。在每只动物的每种浓度下，将这四种化学物质按照它们的 LB/MB 比大小排序，再按照它们的潜伏期差异排序。无论浓度如何，相同的化学物质在不同动物中都处于相同的排序。此外，对于每种化学物质，比值和潜伏期差异的大小仅随浓度有微小变化。因此，整个黏膜上相对反应幅度和潜伏期差异的时空模式以不同方式表征了气味剂。这样的时空编码，结合物理化学因素，表明鼻子以类似于气相色谱仪的方式分离蒸汽。先前观察到的随着气流方向通过嗅囊的反转，比值模式也发生反转，进一步支持了这一点。
