van den Broek M J, Mossel D A, Eggenkamp A E
Appl Environ Microbiol. 1979 Mar;37(3):438-42. doi: 10.1128/aem.37.3.438-442.1979.
A study was carried out on the occurrence of Vibrio parahaemolyticus in Dutch mussels originating from the East Schelde Estuary. In a totol of 79 10-g tissue samples, 3 (3.8%) were found to contain V. parahaemolyticus. In a second survey, 6 out of 23 bags of mussels (26%) contained one or more strains of V. parahaemolyticus in 5-g tissue samples. The many limitations of current methodology used in such surveys are stressed. Positive samples can be missed because viable cells may die during refrigerated transport. Surviving cells also may not be detected because they have been sublethally stressed. In addition, the unreliability of the identification criterion of no growth in 10% NaCl was demonstrated.
对源自东斯海尔德河口的荷兰贻贝中副溶血性弧菌的发生情况进行了一项研究。在总共79个10克组织样本中,发现3个(3.8%)含有副溶血性弧菌。在第二项调查中,23袋贻贝中有6袋(26%)在5克组织样本中含有一种或多种副溶血性弧菌菌株。强调了此类调查中当前使用方法的许多局限性。由于活细胞可能在冷藏运输过程中死亡,阳性样本可能会被遗漏。存活的细胞也可能未被检测到,因为它们受到了亚致死性应激。此外,还证明了在10%氯化钠中不生长的鉴定标准不可靠。