Forman J, Britton S
J Exp Med. 1973 Feb 1;137(2):369-86. doi: 10.1084/jem.137.2.369.
The cytotoxic effect of spleen cells from H-2 allogeneic mice was tested in vitro against an A strain leukemia (YAC) labeled with [(125)I]iododeoxyuridine. After the mice were primed with tumor cells, significant and specific H-2 immunity was detected on day 3 and peak cytotoxicity was observed between 7 and 14 days after priming. Two effector cells appear to be involved in the host response, because spleens taken from mice soon after priming were not sensitive to antitheta sera and complement while those taken during the peak stages of the response showed a marked reduction in cytotoxicity after treatment. Macrophages were not involved, since removal of these cells by the carbonyl iron method did not result in any reduction in cytotoxicity. Immune serum that was capable of inducing cell-mediated cytotoxicity in normal spleen cell populations also augmented cytotoxicity of spleen cells taken from mice primed 3 days previously. However, when spleen cells were taken from mice during the peak phase of the immune response, the same serum at the same dilutions inhibited the preexisting cytotoxicity. A difference was also detected in the killing efficiencies between early and late immune cells.
用[（125）I]碘脱氧尿苷标记的A系白血病（YAC）对H-2同种异体小鼠的脾细胞的细胞毒性作用进行了体外测试。在用肿瘤细胞致敏小鼠后，在第3天检测到显著且特异性的H-2免疫，并且在致敏后7至14天观察到细胞毒性峰值。似乎有两种效应细胞参与宿主反应，因为致敏后不久从小鼠取出的脾脏对抗θ血清和补体不敏感，而在反应高峰期取出的脾脏在处理后细胞毒性显著降低。巨噬细胞未参与，因为用羰基铁法去除这些细胞不会导致细胞毒性的任何降低。能够在正常脾细胞群体中诱导细胞介导的细胞毒性的免疫血清也增强了取自3天前致敏小鼠的脾细胞的细胞毒性。然而，当在免疫反应高峰期从小鼠取出脾细胞时，相同稀释度的相同血清会抑制预先存在的细胞毒性。早期和晚期免疫细胞之间的杀伤效率也存在差异。
