脂质和盐对双层膜中碳氰染料诱导光电压的影响。

Lipid and salt effects on carbocyanine dye-induced photo-voltages in bilayer membranes.

作者信息

Baker J A, Duchek J R, Hooper R L, Koftan R J, Huebner J S

出版信息

Biochim Biophys Acta. 1979 May 3;553(1):1-10. doi: 10.1016/0005-2736(79)90026-9.

Abstract

Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3' -dimethyl-2,2' -oxacarbocyanine chloride (diO-C1-3-CI), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.

摘要

用吸附染料3,3'-二甲基-2,2'-氧杂羰花青氯（diO-C1-3-CI）对双层膜进行闪光照射所诱导的电压瞬变，会随膜脂质组成和水溶液盐浓度而变化。电压瞬变可能是由光异构化后吸附染料分子的物理运动引起的。水溶液中盐（氯化钠和溴化钠）浓度的增加，通过减少吸附到膜上的染料量以及减小有效膜厚度，降低了光电压幅度。光电压的上升时间和下降时间随盐浓度和膜脂质组成而系统地变化，反映了膜表层的结构变化。