赤藓红与pH梯度诱导双层膜中的光电压。
Erythrosin and pH gradient induced photo-voltages in bilayer membranes.
作者信息
Varnadore W E, Arrieta R T, Duchek J R, Huebner J S
出版信息
J Membr Biol. 1982;65(1-2):147-53. doi: 10.1007/BF01870478.
PMID:6276554
Abstract
Erythrosin and light flashes induce voltage transients across bilayer membranes in the presence of transmembrane pH gradients. Fast voltage transients, which rise in less than 50 nsec and fall in approximately 500 nsec, are attributed to photo-deprotonation of dye sorbed in the glycerol region of phospholipid membranes. Six other halogenated xanthene dyes induce similar effects, which apparently resulted from triplet states of monoanionic dye. No photo-effects were observed with fluorescein.
摘要
在存在跨膜pH梯度的情况下，赤藓红和光脉冲会在双层膜上诱导电压瞬变。快速电压瞬变在不到50纳秒内上升，并在约500纳秒内下降，这归因于吸附在磷脂膜甘油区域的染料的光去质子化。其他六种卤代呫吨染料也会产生类似的效应，这显然是由单阴离子染料的三重态引起的。用荧光素未观察到光效应。
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