Arai K, Yasuda J
Jpn J Med Sci Biol. 1978 Apr;31(2):105-18. doi: 10.7883/yoken1952.31.105.
The agglutination test with latex particles coated with aggregated human IgG was introduced into the evaluation of Coombs serum as an additional test for anti-IgG antibody activity. In Coombs sera prepared by the conventional immunization method employing Freund's adjuvant, latex agglutination titers were found much lower than those of anti-D-coated red cell agglutination. On the other hand, in sera prepared by other immunization methods, such as the one according to Haynes and Chaplin (1971), anti-IgG antibody response was readily observed by IgG-coated latex agglutination. Specificity of anti-IgG antibodies in the latter sera seems to be predominantly directed to aggregated human IgG.
将包被人聚合IgG的乳胶颗粒凝集试验引入抗人球蛋白血清的评估中，作为抗IgG抗体活性的一项附加检测。在用弗氏佐剂的传统免疫方法制备的抗人球蛋白血清中，乳胶凝集效价远低于抗-D包被红细胞凝集效价。另一方面，在用其他免疫方法（如Haynes和Chaplin（1971）所述方法）制备的血清中，通过IgG包被乳胶凝集很容易观察到抗IgG抗体反应。后一种血清中抗IgG抗体的特异性似乎主要针对人聚合IgG。
