Graham E F, Crabo B G, Pace M M
J Anim Sci. 1978;47 Suppl 2:80-119.
From the studies cited it was concluded that short and long term preservation of stallion semen has encountered major obstacles. Fertilizing capacity of extended or extended and cooled spermatozoa has been impaired. With the hydrogen ion extenders, the fertility was depressed either with or without glycerol when the semen was inseminated immediately after extension. With the cream-gel extender, fertility was not impaired when inseminated immediately after extension, but was impaired after storage at 5 C for 24 hr or in the presence of glycerol. The fertilizing capacity of extended frozen spermatozoa particularly from some stallions has been more adversely affected than that of others. These studies show that the pregnancy rate range was from 50 to 80% for raw semen from the same stallion used in the frozen studies. Pregnancy rate with this magnitude of difference must be carefully weighed in applying the results from a few stallions to the population. Sufficient information has been generated to suggest that the preservation of stallion spermatozoa is possible but the fertilizing capacity is impaired. Causes of this impairment must be further investigated. When this is accomplished, the number of motile spermatozoa needed per insemination and the frequency of insemination required for optimal fertilization reported in this review must then be reevaluated.
从所引用的研究中可以得出结论，种马精液的短期和长期保存遇到了主要障碍。稀释后的精子或稀释并冷却后的精子的受精能力受到了损害。对于氢离子稀释剂，当精液在稀释后立即输精时，无论有无甘油，其生育力都会降低。对于乳膏 - 凝胶稀释剂，在稀释后立即输精时生育力未受损害，但在5℃下储存24小时或存在甘油时生育力会受到损害。稀释后的冷冻精子，尤其是来自某些种马的精子，其受精能力受到的不利影响比其他种马更大。这些研究表明，在冷冻研究中使用的来自同一匹种马的新鲜精液的妊娠率范围为50%至80%。在将少数种马的结果应用于整个种群时，必须仔细权衡这种差异程度的妊娠率。已经产生了足够的信息表明种马精子的保存是可能的，但受精能力受到了损害。这种损害的原因必须进一步研究。当完成这项研究后，本综述中报道的每次输精所需的活动精子数量以及最佳受精所需的输精频率必须重新评估。
