Alvarenga M A, Papa F O, Landim-Alvarenga F C, Medeiros A S L
Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine, São Paulo State University, UNESP-Botucatu, SP 18618-000, Brazil.
Anim Reprod Sci. 2005 Oct;89(1-4):105-13. doi: 10.1016/j.anireprosci.2005.07.001.
Stallion semen cryopreservation, despite its impact on the horse industry, is not an established technology. During the last years, a number of modifications have been proposed to the freezing process, however, a large population of stallions still have poor semen quality and fertility after frozen-thawed. Glycerol toxicity could be a reason for the variation on stallion sperm freezability. There are limited publications concerning the use of alternative cryoprotectants for equine sperm. Glycerol is contraceptive for some species and other cryoprotectors, such as amides, have been show to be a good option for freezing semen of these species. Recent reports have shown encouraging data respecting the use of amides as cryoprotectants for stallions, with more remarkable improvements for semen from stallions that freeze poorly when glycerol is used.
种马精液冷冻保存技术尽管对养马业有重要影响，但仍未成为一项成熟的技术。在过去几年中，人们对冷冻过程提出了一些改进措施，然而，仍有大量种马在冻融后精液质量和生育能力较差。甘油毒性可能是种马精子冷冻能力存在差异的一个原因。关于使用替代冷冻保护剂保存马精子的文献有限。甘油对某些物种具有避孕作用，而其他冷冻保护剂，如酰胺类，已被证明是冷冻这些物种精液的良好选择。最近的报告显示，有关使用酰胺类作为种马冷冻保护剂的数据令人鼓舞，对于使用甘油冷冻效果不佳的种马的精液，使用酰胺类冷冻保护剂有更显著的改善。
