3'-脱氧腺苷（虫草素）对从分离的SV40-3T3细胞核中流出核糖核酸的抑制作用。
Inhibition of ribonucleic acid efflux from isolated SV40-3T3 cell nuclei by 3'-deoxyadenosine (cordycepin).
作者信息
Agutter P S, McCaldin B
出版信息
Biochem J. 1979 May 15;180(2):371-8. doi: 10.1042/bj1800371.
Abstract
The effect of 3'-deoxyadenosine (cordycepin) on mRNA efflux from isolated SV40-3T3 cell nuclei has been studied and compared with its effect on the nucleoside triphosphatase activity in the isolated nuclear envelope. Inhibition of mRNA efflux occurs rapidly, but is dependent on the presence of ATP. Half-maximal inhibition occurs with 40 microM-cordycepin. The effect is not simulated by 2'-deoxyadenosine or by actinomycin D, and adenosine provides a substantial degree of protection against it. Cordycepin does not directly inhibit the nucleoside triphosphatase. The stimulation of this enzyme by poly(A) is not affected unless the poly(A) and cordycepin are incubated together with nuclear lysate in the presence of ATP; in this case the stimulation is significantly reduced. Possible interpretations of these results and their relevance for understanding the system in vivo for nucleo-cytoplasmic messenger transport are discussed.
摘要
研究了3'-脱氧腺苷（虫草素）对从分离的SV40 - 3T3细胞核中mRNA流出的影响，并将其与对分离的核膜中核苷三磷酸酶活性的影响进行了比较。mRNA流出的抑制迅速发生，但依赖于ATP的存在。40微摩尔虫草素可产生半数最大抑制。2'-脱氧腺苷或放线菌素D不会模拟这种效应，腺苷可提供相当程度的保护。虫草素不会直接抑制核苷三磷酸酶。除非在ATP存在的情况下将聚腺苷酸（poly(A)）和虫草素与核裂解物一起孵育，否则poly(A)对该酶的刺激不受影响；在这种情况下，刺激会显著降低。讨论了这些结果的可能解释及其对理解体内核质信使运输系统的相关性。
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