大肠杆菌中异亮氨酸生物合成的替代途径。
Alternate pathway for isoleucine biosynthesis in Escherichia coli.
作者信息
Phillips A T, Nuss J I, Moosic J, Foshay C
出版信息
J Bacteriol. 1972 Feb;109(2):714-9. doi: 10.1128/jb.109.2.714-719.1972.
Abstract
A threonine dehydrataseless mutant of Escherichia coli, Crookes strain, was observed to grow on an acetate minimal medium without the usual requirement for isoleucine supplementation. Both the wild-type Crookes strain and a threonine auxotroph metabolized l-glutamate-1-(14)C to l-isoleucine-1-(14)C with no appreciable randomization, suggesting that a pathway for isoleucine formation from glutamate via beta-methylaspartate, beta-methyloxaloacetate, and alpha-ketobutyrate was possible in addition to the pathway from threonine and alpha-ketobutyrate. Crude cell-free extracts formed (14)C-beta-methylaspartate from (14)C-glutamate, and the conversion of beta-methylaspartate to alpha-ketobutyrate was also demonstrated, thus supporting the conclusion that glutamate can serve as a precursor of alpha-ketobutyrate (and isoleucine) without the necessary involvement of threonine as an intermediate.
摘要
观察到大肠杆菌克鲁克斯菌株的一种无苏氨酸脱水酶突变体能够在乙酸盐基本培养基上生长，而无需像往常那样添加异亮氨酸。野生型克鲁克斯菌株和苏氨酸营养缺陷型菌株都能将L-谷氨酸-1-(14)C代谢为L-异亮氨酸-1-(14)C，且没有明显的随机化现象，这表明除了从苏氨酸和α-酮丁酸形成异亮氨酸的途径外，还可能存在一条从谷氨酸经β-甲基天冬氨酸、β-甲基草酰乙酸和α-酮丁酸形成异亮氨酸的途径。粗制的无细胞提取物能从(14)C-谷氨酸形成(14)C-β-甲基天冬氨酸，并且还证实了β-甲基天冬氨酸向α-酮丁酸的转化，从而支持了谷氨酸可以作为α-酮丁酸（和异亮氨酸）的前体而无需苏氨酸作为中间产物参与的结论。
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本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE FORMATION OF ISOLEUCINE FROM BETA-METHYLASPARTIC ACID IN ESCHERICHIA COLI W.大肠杆菌W中由β-甲基天冬氨酸合成异亮氨酸
J Biol Chem. 1965 Jul;240:2971-5.
4
THE GLUTAMATE MUTASE SYSTEM. ASSAYS AND PROPERTIES.谷氨酸变位酶系统。分析方法与特性。
J Biol Chem. 1964 Oct;239:3260-6.
5
Rapid sensitive method for determining H3-water in body fluids by liquid scintillation spectrometry.通过液体闪烁光谱法测定体液中H3-水的快速灵敏方法。
Proc Soc Exp Biol Med. 1959 Oct;102:8-12. doi: 10.3181/00379727-102-25125.
6
Pathway of ribose 5-phosphate utilization in Microbacterium lacticum.乳酸微杆菌中5-磷酸核糖的利用途径。
J Bacteriol. 1961 Feb;81(2):246-57. doi: 10.1128/jb.81.2.246-257.1961.
7
Glutamate biosynthesis in Acetobacter suboxydans. VI. Formation from acetate plus pyruvate.弱氧化醋杆菌中谷氨酸的生物合成。VI. 由乙酸盐和丙酮酸生成。
Biochemistry. 1966 Aug;5(8):2646-53. doi: 10.1021/bi00872a024.
8
Metabolism of beta-methylaspartate by a pseudomonad.假单胞菌对β-甲基天冬氨酸的代谢
J Bacteriol. 1968 Dec;96(6):2094-8. doi: 10.1128/jb.96.6.2094-2098.1968.
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