Darling S, Theilade J, Birch-Andersen A
J Bacteriol. 1972 Apr;110(1):336-45. doi: 10.1128/jb.110.1.336-345.1972.
Cells of Saccharomyces cerevisiae and Hansenula anomala were digested with snail enzyme under conditions yielding prospheroplasts. Surrounding envelopes were isolated after lysis of prospheroplasts in distilled water. The envelope material was embedded and sectioned for electron microscopy, and thin, hollow structures still retaining the elongated form of the original cells were seen. The envelopes were of low electron density in sections stained with uranyl magnesium acetate and lead citrate, but were more electron-dense when stained with phosphotungstic acid. Shadowed preparations of prospheroplast envelopes revealed structures resembling ghosts. These "ghosts" were similar to the original cells in form and size but seemed to be very thin. Varying numbers of anular structures (bud scars) were found on them. Chemical analyses of the envelope indicated that an alkali-soluble glucan was a major constituent. The results show that the prospheroplast envelope is part of the original cell wall of the yeast and is located in close apposition to the cytoplasmic membrane.
在能产生原生质球的条件下，用蜗牛酶消化酿酒酵母和异常汉逊酵母的细胞。原生质球在蒸馏水中裂解后，分离出其周围的包膜。将包膜材料包埋并切片用于电子显微镜观察，可见仍保留原始细胞细长形态的薄而中空的结构。在用醋酸铀镁和柠檬酸铅染色的切片中，包膜的电子密度较低，但在用磷钨酸染色时，电子密度更高。原生质球包膜的投影制备显示出类似幽灵的结构。这些“幽灵”在形态和大小上与原始细胞相似，但似乎非常薄。在它们上面发现了数量不等的环状结构（芽痕）。对包膜的化学分析表明，一种碱溶性葡聚糖是主要成分。结果表明，原生质球包膜是酵母原始细胞壁的一部分，与细胞质膜紧密相邻。
