Fleet G H, Manners D J
J Gen Microbiol. 1976 May;94(1):180-92. doi: 10.1099/00221287-94-1-180.
An alkali-soluble glucan was obtained from the cell walls of Saccharomyces cerevisiae NCYC1109 and baker's yeast by extraction with cold, dilute sodium hydroxide under nitrogen. The glucan, which represented approximately 20% of purified glucan was homogeneous and was shown to be free from contamination by other cell-wall polysaccharides by ultracentrifuging, gel filtration and electrophoresis. In addition to glucose, the glucan contained traces of mannose and nitrogen, but no hexosamine. Structural analyses revealed the presence of 80-85% (1 leads to 3)-beta-D linkages, 8-12% (1 leads to 6)-beta-D linkages and 3-4% branched residues linked through C-1, C-3 and C-6. The molecular weight of the glucan was estimated to be about 250000. Electron-microscopic examination of the cell walls after alkali extraction showed that an amorphous surface layer had been removed revealing numerous bud scar structures.
在氮气保护下，用冷的稀氢氧化钠溶液从酿酒酵母NCYC1109和面包酵母的细胞壁中提取出一种碱溶性葡聚糖。这种葡聚糖约占纯化葡聚糖的20%，性质均一，通过超速离心、凝胶过滤和电泳证明其未被其他细胞壁多糖污染。除葡萄糖外，该葡聚糖还含有微量甘露糖和氮，但不含己糖胺。结构分析表明，该葡聚糖含有80 - 85%的（1→3）-β-D糖苷键、8 - 12%的（1→6）-β-D糖苷键以及3 - 4%通过C-1、C-3和C-6连接的分支残基。该葡聚糖的分子量估计约为250000。碱提取后对细胞壁进行电子显微镜检查发现，无定形表面层已被去除，露出许多芽痕结构。
