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一种用于大肠杆菌、肺炎克雷伯菌和气杆菌的多价噬菌体的分离。

Isolation of a polyvalent bacteriophage for Escherichia coli, Klebsiella pneumoniae, and Aerobacter aerogenes.

作者信息

Souza K A, Ginoza H S, Haight R D

出版信息

J Virol. 1972 May;9(5):851-6. doi: 10.1128/JVI.9.5.851-856.1972.

DOI:10.1128/JVI.9.5.851-856.1972
PMID:4554466
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356383/
Abstract

A lytic bacteriophage isolated from sewage was found to attack strains of Aerobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae, but not members of the genera Salmonella, Proteus, and Serratia. The phage, designated phimp, contained deoxyribonucleic acid with a 50% guanine plus cytosine ratio and a molecular weight of 23.1 x 10(6) daltons. Single-step growth experiments of phimp plated at 37 C on A. aerogenes A2 gave a mean latent period of 20 min, an average burst size of 103 plaque-forming units/infected cell, and an average adsorption rate constant of 3 x 10(-10) ml/min. Electron microscopy of phimp revealed a phage with a flexible tail (165 nm long and 6 nm wide). The phage head had a hexagonal outline (62 nm in diameter).

摘要

从污水中分离出的一种裂解性噬菌体被发现可攻击产气气杆菌、大肠杆菌和肺炎克雷伯菌菌株,但不攻击沙门氏菌属、变形杆菌属和沙雷氏菌属的成员。该噬菌体被命名为phimp,其脱氧核糖核酸中鸟嘌呤加胞嘧啶的比例为50%,分子量为23.1×10⁶道尔顿。phimp在37℃下接种于产气气杆菌A2上进行的一步生长实验,平均潜伏期为20分钟,平均裂解量为每感染细胞103个噬菌斑形成单位,平均吸附速率常数为3×10⁻¹⁰毫升/分钟。phimp的电子显微镜观察显示,该噬菌体有一条可弯曲的尾巴(长165纳米,宽6纳米)。噬菌体头部呈六边形轮廓(直径62纳米)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f4c/356383/4ebd590ebbc1/jvirol00269-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f4c/356383/4ebd590ebbc1/jvirol00269-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f4c/356383/4ebd590ebbc1/jvirol00269-0143-a.jpg

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