Talmadge J E, Uithoven K A, Reif A E
Clin Exp Metastasis. 1985 Jan-Mar;3(1):61-72. doi: 10.1007/BF01758954.
The purpose of these studies was to determine whether the high macrophage content (greater than 50 per cent) of the 90Sr-induced osteogenic sarcoma J (Os-J) of recent origin correlated with its immunogenicity or low metastatic potential. Cloning experiments demonstrated that the Os-J tumor is heterogeneous with regard to the production of experimental pulmonary metastases. Immunization-challenge studies in syngeneic mice and comparisons of tumor growth in normal or nude mice established that the slow growing Os-J tumor is poorly immunogenic. In vitro studies demonstrated that the Os-J tumor is highly susceptible to macrophages-mediated lysis. This may explain the slow growth of the tumor in normal recipients with an intact mononuclear phagocyte system, as compared with the more rapid emergence of tumors in macrophage-suppressed mice. However, spontaneous metastases of the Os-J tumor were not observed either in normal or macrophage-suppressed mice. Although a high macrophage infiltration of neoplasms could slow tumor growth, this was not associated with the immunogenicity of the neoplasm and did not appear to limit the spontaneous metastasis of this essentially benign neoplasm.
这些研究的目的是确定近期起源的90Sr诱导的骨肉瘤J（Os-J）中高巨噬细胞含量（超过50%）是否与其免疫原性或低转移潜能相关。克隆实验表明，Os-J肿瘤在实验性肺转移的产生方面具有异质性。在同基因小鼠中的免疫攻击研究以及正常或裸鼠中肿瘤生长的比较表明，生长缓慢的Os-J肿瘤免疫原性较差。体外研究表明，Os-J肿瘤对巨噬细胞介导的裂解高度敏感。这可能解释了与巨噬细胞抑制的小鼠中肿瘤更快出现相比，在具有完整单核吞噬细胞系统的正常受体中肿瘤生长缓慢的原因。然而，在正常或巨噬细胞抑制的小鼠中均未观察到Os-J肿瘤的自发转移。尽管肿瘤中高巨噬细胞浸润可能会减缓肿瘤生长，但这与肿瘤的免疫原性无关，并且似乎并未限制这种基本良性肿瘤的自发转移。
