Zuccarelli A J, Benbow R M, Sinsheimer R L
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1905-10. doi: 10.1073/pnas.69.7.1905.
Mutants of bacteriophage varphiX174 have been isolated that are less dense than wild-type varphiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement varphiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the varphiX genome in the region of cistron E.
已分离出噬菌体φX174的突变体，其在CsCl中的密度低于野生型φX颗粒。当突变体病毒（+）链DNA与野生型互补（-）链DNA杂交时，产生的双链分子具有野生型缺失异源双链体特有的单链环。突变体噬菌体不能在顺反子E中对φX琥珀突变体进行互补。我们得出结论，突变体病毒在顺反子E区域缺失了约7%的φX基因组。
