Benbow R M, Zuccarelli A J, Davis G C, Sinsheimer R L
J Virol. 1974 Apr;13(4):898-907. doi: 10.1128/JVI.13.4.898-907.1974.
Genetic recombination in bacteriophage phiX174 usually takes place early in the infection process and involves two parental replicative form (double-stranded) DNA molecules. The host recA protein is required; none of the nine known phiX174 cistron products is essential. The products of a single recombination event are nonreciprocal and asymmetric. Typically, only one of the parental genotypes and one recombinant genotype are recovered from a single cell. An alternative, less efficient recombination mechanism which requires an active phiX174 cistron A protein is observed in the absence of the host recA gene product.
噬菌体φX174中的基因重组通常发生在感染过程的早期,涉及两个亲本复制型(双链)DNA分子。宿主recA蛋白是必需的;已知的9个φX174顺反子产物中没有一个是必不可少的。单次重组事件的产物是非互惠且不对称的。通常,从单个细胞中只能回收一种亲本基因型和一种重组基因型。在没有宿主recA基因产物的情况下,会观察到另一种效率较低的重组机制,该机制需要有活性的φX174顺反子A蛋白。