大肠杆菌DNA聚合酶II缺陷型突变体的分离与部分特性分析
Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II.
作者信息
Campbell J L, Soll L, Richardson C C
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090-4. doi: 10.1073/pnas.69.8.2090.
Abstract
A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors. The polA1 mutation was suppressed during mutagenesis by introduction of the suppressor, su7(+), into the parental strain. The mutant, HMS83 polA1 polB1, contains less than 0.5% of the normal levels of DNA polymerase II. The only polymerase activity detected in the mutant is DNA polymerase III. E. coli HMS83 grows normally at both 25 and 42 degrees , and supports the growth of bacteriophages T4, T7, lambda, varphiX174, 186, P2, and fd. The polB mutation does not affect sensitivity to ultraviolet irradiation or recombination frequencies.
摘要
通过用N-甲基-N'-硝基-N-亚硝基胍诱变大肠杆菌polA1并检测存活菌提取物中的聚合酶活性，已从大肠杆菌中分离出一种DNA聚合酶II缺陷型突变体。在诱变过程中，通过将抑制基因su7(+)导入亲代菌株，polA1突变被抑制。该突变体HMS83 polA1 polB1所含的DNA聚合酶II水平低于正常水平的0.5%。在该突变体中检测到的唯一聚合酶活性是DNA聚合酶III。大肠杆菌HMS83在25℃和42℃时均能正常生长，并能支持噬菌体T4、T7、λ、φX174、186、P2和fd的生长。polB突变不影响对紫外线照射的敏感性或重组频率。
相似文献
1
Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090-4. doi: 10.1073/pnas.69.8.2090.
2
A mutant of Escherichia coli defective in DNA polymerase II activity.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3238-42. doi: 10.1073/pnas.69.11.3238.
3
Repair of DNA damaged by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli.
Mutat Res. 1967 May-Jun;4(3):369-71. doi: 10.1016/0027-5107(67)90031-0.
4
Study of the properties of a DNA-polymerase-deficient mutant of Escherichia coli.
Sov Genet. 1974 Jan 1;7(9):1242-4.
5
Mutations induced in Haemophilus influenzae by transformation with nitrosoguanidine-treated DNA.
Mutat Res. 1972 Feb;14(2):137-46. doi: 10.1016/0027-5107(72)90042-5.
6
Comparative analysis of deletion and base-change mutabilities of Escherichia coli B strains differing in DNA repair capacity (wild-type, uvrA-, polA-, recA-) by various mutagens.
Mutat Res. 1975 Jan;27(1):27-44. doi: 10.1016/0027-5107(75)90271-7.
7
8
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
9
Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
J Bacteriol. 1974 Oct;120(1):407-15. doi: 10.1128/jb.120.1.407-415.1974.
10
Evidence that pretreatment of Escherichia coli cells with N-methyl-N'-nitro-N-nitrosoguanidine enhances mutability of subsequently infecting phage lambda.
Mol Gen Genet. 1973 Nov 22;126(4):319-24. doi: 10.1007/BF00269441.
引用本文的文献
1
Escherichia coli DnaE Polymerase Couples Pyrophosphatase Activity to DNA Replication.
PLoS One. 2016 Apr 6;11(4):e0152915. doi: 10.1371/journal.pone.0152915. eCollection 2016.
2
Exchange between Escherichia coli polymerases II and III on a processivity clamp.
Nucleic Acids Res. 2016 Feb 29;44(4):1681-90. doi: 10.1093/nar/gkv1375. Epub 2015 Dec 10.
3
Harnessing synthetic lethal interactions in anticancer drug discovery.
Nat Rev Drug Discov. 2011 May;10(5):351-64. doi: 10.1038/nrd3374.
4
Interplay of DNA repair, homologous recombination, and DNA polymerases in resistance to the DNA damaging agent 4-nitroquinoline-1-oxide in Escherichia coli.
DNA Repair (Amst). 2010 Oct 5;9(10):1090-7. doi: 10.1016/j.dnarep.2010.07.008. Epub 2010 Aug 19.
5
DNA polymerase II (polB) is involved in a new DNA repair pathway for DNA interstrand cross-links in Escherichia coli.
J Bacteriol. 1999 May;181(9):2878-82. doi: 10.1128/JB.181.9.2878-2882.1999.
6
Absence of a role for DNA polymerase II in SOS-induced translesion bypass of phi X174.
J Bacteriol. 1993 Jan;175(2):561-4. doi: 10.1128/jb.175.2.561-564.1993.
7
Involvement of Escherichia coli DNA polymerase II in response to oxidative damage and adaptive mutation.
J Bacteriol. 1994 Oct;176(20):6221-8. doi: 10.1128/jb.176.20.6221-6228.1994.
8
Characterization of long patch excision repair of DNA in ultraviolet-irradiated Escherichia coli: an inducible function under rec-lex control.
Mol Gen Genet. 1982;185(2):189-97. doi: 10.1007/BF00330785.
9
Novel histone H2A-like protein of escherichia coli.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5097-101. doi: 10.1073/pnas.77.9.5097.
10
Evidence that a high molecular weight replicative DNA polymerase is conserved during evolution.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6771-5. doi: 10.1073/pnas.78.11.6771.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.
Biochim Biophys Acta. 1963 Jun 25;72:290-7.
3
4
Genetic variation in the sex factor of Escherichia coli.
J Bacteriol. 1960 Mar;79(3):321-30. doi: 10.1128/jb.79.3.321-330.1960.
5
Acetylornithinase of Escherichia coli: partial purification and some properties.
J Biol Chem. 1956 Jan;218(1):97-106.
6
Transduction of linked genetic characters of the host by bacteriophage P1.
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
7
Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination.
Genetics. 1966 Jun;53(6):1137-50. doi: 10.1093/genetics/53.6.1137.
8
Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.
Proc Natl Acad Sci U S A. 1971 May;68(5):1002-5. doi: 10.1073/pnas.68.5.1002.
9
Escherichia coli mutants temperature-sensitive for DNA synthesis.
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
10
Function of DNA polymerase 3 in DNA replication.
Nat New Biol. 1971 Dec 29;234(52):285-6. doi: 10.1038/newbio234285a0.
Zotero 插件