大肠杆菌DNA聚合酶II缺陷型突变体的分离与部分特性分析
Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II.
作者信息
Campbell J L, Soll L, Richardson C C
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090-4. doi: 10.1073/pnas.69.8.2090.
Abstract
A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors. The polA1 mutation was suppressed during mutagenesis by introduction of the suppressor, su7(+), into the parental strain. The mutant, HMS83 polA1 polB1, contains less than 0.5% of the normal levels of DNA polymerase II. The only polymerase activity detected in the mutant is DNA polymerase III. E. coli HMS83 grows normally at both 25 and 42 degrees , and supports the growth of bacteriophages T4, T7, lambda, varphiX174, 186, P2, and fd. The polB mutation does not affect sensitivity to ultraviolet irradiation or recombination frequencies.
摘要
通过用N-甲基-N'-硝基-N-亚硝基胍诱变大肠杆菌polA1并检测存活菌提取物中的聚合酶活性，已从大肠杆菌中分离出一种DNA聚合酶II缺陷型突变体。在诱变过程中，通过将抑制基因su7(+)导入亲代菌株，polA1突变被抑制。该突变体HMS83 polA1 polB1所含的DNA聚合酶II水平低于正常水平的0.5%。在该突变体中检测到的唯一聚合酶活性是DNA聚合酶III。大肠杆菌HMS83在25℃和42℃时均能正常生长，并能支持噬菌体T4、T7、λ、φX174、186、P2和fd的生长。polB突变不影响对紫外线照射的敏感性或重组频率。
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