对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.
作者信息
Gefter M L, Hirota Y, Kornberg T, Wechsler J A, Barnoux C
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
Abstract
A series of double mutants carrying one of the thermosensitive mutations for DNA synthesis (dnaA, B, C, D, E, F, and G) and the polA1 mutation of DeLucia and Cairns, were constructed. Enzyme activities of DNA Polymerases II and III were measured in each mutant. DNA Polymerase II activity was normal in all strains tested. DNA Polymerase III activity is thermosensitive specifically in those strains having thermoscnsitive mutations at the dnaE locus. From these results we conclude that DNA Polymerases II and III are independent enzymes and that DNA Polymerase III is an enzyme required for DNA replication in Escherichia coli.
摘要
构建了一系列双突变体，这些双突变体带有一个用于DNA合成的温度敏感突变（dnaA、B、C、D、E、F和G）以及DeLucia和Cairns的polA1突变。在每个突变体中测量了DNA聚合酶II和III的酶活性。在所有测试菌株中，DNA聚合酶II活性正常。DNA聚合酶III活性在那些在dnaE位点具有温度敏感突变的菌株中表现出特异性温度敏感性。从这些结果我们得出结论，DNA聚合酶II和III是独立的酶，并且DNA聚合酶III是大肠杆菌中DNA复制所需的酶。
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