一种DNA聚合酶II活性存在缺陷的大肠杆菌突变体。
A mutant of Escherichia coli defective in DNA polymerase II activity.
作者信息
Hirota Y, Gefter M, Mindich L
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3238-42. doi: 10.1073/pnas.69.11.3238.
Abstract
A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41 degrees as well as at 30 degrees . All bacteriophages, F factors, and R factors examined so far grow normally in the mutant. Sensitivity of the mutant to ultraviolet radiation and alkylating reagents in growth media was the same as that of the wild type. The mutation, polB1, is recessive with respect to wild type. The polB1 mutation can coexist with recombination-defective mutations. Genetic mapping studies show the mutation to be located at about 2 min on the E. coli map.
摘要
分离出了一种DNA聚合酶II活性有缺陷的大肠杆菌突变体。提取物的DNA聚合酶活性约为正常活性的0.1%。分析了该突变对细胞复制各种来源DNA能力的影响。突变细菌在41摄氏度和30摄氏度下均能正常生长。到目前为止所检测的所有噬菌体、F因子和R因子在该突变体中均能正常生长。突变体在生长培养基中对紫外线辐射和烷基化试剂的敏感性与野生型相同。polB1突变相对于野生型是隐性的。polB1突变可与重组缺陷型突变共存。遗传图谱研究表明该突变位于大肠杆菌图谱上约2分钟处。
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