不饱和脂肪酸生物合成停止后大肠杆菌中甲基半乳糖苷转运的抑制作用
Inhibition of methylgalactoside transport in Escherichia coli upon the cessation of unsaturated fatty acid biosynthesis.
作者信息
Robbins A R, Rotman B
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2125-9. doi: 10.1073/pnas.69.8.2125.
Abstract
The activity of the methylgalactoside transport system of E. coli is impaired upon treatment with 3-decynoyl-N-acetylcysteamine, an inhibitor of unsaturated fatty-acid synthesis. Treated cells are unable to be induced for permease activity, while transport sites synthesized before treatment show a regular loss of activity. The inhibition of methylgalactoside transport occurs at a step after translation of the galactose-binding protein, a component of the permease, and appears to be highly specific, since drug-treated cells show normal viability, protein synthesis, and membrane integrity when transport activity is greatly reduced. A second transport system, the galactoside permease, shows significantly less sensitivity to the inhibitor. That the activity of this permease is maintained in the presence of this inhibitor suggests that the inhibitor does not impair energy coupling.
摘要
用3-癸炔酰-N-乙酰半胱胺（一种不饱和脂肪酸合成抑制剂）处理后，大肠杆菌甲基半乳糖苷转运系统的活性受损。经处理的细胞无法被诱导产生通透酶活性，而在处理前合成的转运位点则呈现出活性的规律性丧失。甲基半乳糖苷转运的抑制发生在通透酶组分半乳糖结合蛋白翻译后的一个步骤，并且似乎具有高度特异性，因为当转运活性大幅降低时，经药物处理的细胞仍表现出正常的活力、蛋白质合成和膜完整性。第二个转运系统，即半乳糖苷通透酶，对该抑制剂的敏感性明显较低。在该抑制剂存在的情况下这种通透酶的活性得以维持，这表明该抑制剂不会损害能量偶联。
相似文献
1
Inhibition of methylgalactoside transport in Escherichia coli upon the cessation of unsaturated fatty acid biosynthesis.不饱和脂肪酸生物合成停止后大肠杆菌中甲基半乳糖苷转运的抑制作用
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2125-9. doi: 10.1073/pnas.69.8.2125.
2
The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12.大肠杆菌K12的β-甲基半乳糖苷转运系统和半乳糖结合蛋白的调控
Eur J Biochem. 1971 Apr 30;19(4):457-70. doi: 10.1111/j.1432-1033.1971.tb01336.x.
3
Site of energy coupling in the carrier mechanism for beta-galactoside transport.β-半乳糖苷转运载体机制中的能量偶联位点。
Biochim Biophys Acta. 1971 Mar 9;233(1):176-88. doi: 10.1016/0005-2736(71)90370-1.
4
Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities.大肠杆菌中半乳糖和半乳糖苷的转运系统。II. 底物和诱导剂特异性。
J Mol Biol. 1968 Sep 14;36(2):247-60. doi: 10.1016/0022-2836(68)90379-3.
5
A new spin-labelled substrate for -galactosidase and -galactoside permease.一种用于β-半乳糖苷酶和β-半乳糖苷通透酶的新型自旋标记底物。
Biochem Biophys Res Commun. 1972 Dec 18;49(6):1631-7. doi: 10.1016/0006-291x(72)90529-3.
6
Regulation of the -methylgalactoside transport system and the galatose-binding protein by the cell cycle of Escherichia coli.大肠杆菌细胞周期对α-甲基半乳糖苷转运系统和半乳糖结合蛋白的调控。
Proc Natl Acad Sci U S A. 1973 May;70(5):1481-5. doi: 10.1073/pnas.70.5.1481.
7
2-Deoxy-D-galactose, a substrate for the galactose-transport system of Escherichia coli.2-脱氧-D-半乳糖，大肠杆菌半乳糖转运系统的一种底物。
Biochem J. 1977 Oct 15;168(1):15-22. doi: 10.1042/bj1680015.
8
Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease.大肠杆菌中半乳糖和半乳糖苷的转运系统。I.甲基半乳糖苷通透酶的遗传测定与调控
J Mol Biol. 1966 Mar;16(1):42-50. doi: 10.1016/s0022-2836(66)80261-9.
9
Membrane translocation of mannitol in Escherichia coli without phosphorylation.甘露醇在大肠杆菌中无需磷酸化的膜转运。
J Bacteriol. 1973 May;114(2):723-8. doi: 10.1128/jb.114.2.723-728.1973.
10
Energy coupling in the lactose transport system of Escherichia coli.大肠杆菌乳糖转运系统中的能量偶联
Proc Natl Acad Sci U S A. 1970 Jan;65(1):63-9. doi: 10.1073/pnas.65.1.63.
引用本文的文献
1
Regulation of macromolecular biosynthesis in a mutant of Escherichia coli defective in membrane phospholipid biosynthesis.大肠杆菌膜磷脂生物合成缺陷突变体中大分子生物合成的调控。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):385-9. doi: 10.1073/pnas.70.2.385.
2
Regulation of the -methylgalactoside transport system and the galatose-binding protein by the cell cycle of Escherichia coli.大肠杆菌细胞周期对α-甲基半乳糖苷转运系统和半乳糖结合蛋白的调控。
Proc Natl Acad Sci U S A. 1973 May;70(5):1481-5. doi: 10.1073/pnas.70.5.1481.
3
Physical properties of membrane lipids: biological relevance and regulation.膜脂的物理性质：生物学相关性与调控
Bacteriol Rev. 1975 Sep;39(3):232-56. doi: 10.1128/br.39.3.232-256.1975.
4
Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12.大肠杆菌K12甲基半乳糖苷转运系统存在不依赖结合蛋白的底物转运的证据。
Proc Natl Acad Sci U S A. 1975 Feb;72(2):423-7. doi: 10.1073/pnas.72.2.423.
5
Inhibition of de novo fatty acid synthesis by the antibiotic cerulenin in Bacillus subtilis: effects on citrate-Mg2+ transport and synthesis of macromolecules.抗生素浅蓝菌素对枯草芽孢杆菌从头脂肪酸合成的抑制作用：对柠檬酸-Mg2+转运及大分子合成的影响。
Antimicrob Agents Chemother. 1975 Sep;8(3):231-7. doi: 10.1128/AAC.8.3.231.
本文引用的文献
1
GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.大肠杆菌乳糖转运系统膜蛋白组分的遗传控制
Proc Natl Acad Sci U S A. 1967 Mar;57(3):698-705. doi: 10.1073/pnas.57.3.698.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Separate permeases for the accumulation of methyl-beta-D-galactoside and methyl-beta-D-thiogalactoside in Escherichia coli.大肠杆菌中用于积累甲基-β-D-半乳糖苷和甲基-β-D-硫代半乳糖苷的不同通透酶。
Biochim Biophys Acta. 1959 Apr;32:599-601. doi: 10.1016/0006-3002(59)90659-6.
4
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.大肠杆菌碱性磷酸酶的精细结构遗传学与化学研究。I. 碱性磷酸酶的纯化与特性分析
Biochim Biophys Acta. 1960 Mar 11;38:470-83. doi: 10.1016/0006-3002(60)91282-8.
5
Transport of galactose from the inside to the outside of Escherichia coli.半乳糖在大肠杆菌内从内部向外部的转运。
Pathol Biol. 1961 Apr;9:806-10.
6
Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease.大肠杆菌中半乳糖和半乳糖苷的转运系统。I.甲基半乳糖苷通透酶的遗传测定与调控
J Mol Biol. 1966 Mar;16(1):42-50. doi: 10.1016/s0022-2836(66)80261-9.
7
The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12.大肠杆菌K12的β-甲基半乳糖苷转运系统和半乳糖结合蛋白的调控
Eur J Biochem. 1971 Apr 30;19(4):457-70. doi: 10.1111/j.1432-1033.1971.tb01336.x.
8
9
Biogenesis of E. coli membrane: evidence for randomization of lipid phase.大肠杆菌膜的生物合成：脂质相随机化的证据。
Nat New Biol. 1971 Dec 29;234(52):264-7. doi: 10.1038/newbio234264a0.
10
Correlation of in vivo and in vitro phase transitions of membrane lipids in Escherichia coli.大肠杆菌中膜脂体内外相变的相关性
Proc Natl Acad Sci U S A. 1970 Oct;67(2):606-12. doi: 10.1073/pnas.67.2.606.
Zotero 插件