甘露醇在大肠杆菌中无需磷酸化的膜转运。
Membrane translocation of mannitol in Escherichia coli without phosphorylation.
作者信息
Solomon E, Miyal K, Lin E C
出版信息
J Bacteriol. 1973 May;114(2):723-8. doi: 10.1128/jb.114.2.723-728.1973.
Abstract
Galactosyl-mannitol can be transported into cells of Escherichia coli by beta-galactoside permease and can be hydrolyzed rapidly to mannitol and galactose by beta-galactosidase. When a mutant strain lacking enzyme I of the phosphoenolpyruvate phosphotransferase system and constitutive in the lactose system was presented with galactosyl-mannitol in which the mannitol moiety was labeled with (3)H, the liberated mannitol remained inside the cell if the Enzyme II complex of the phosphoenolpyruvate phosphotransferase system for mannitol was uninduced. It is postualted that one of the enzyme II proteins can still catalyze translocation of mannitol across the cell membrane even when phsophorylation is not possible.
摘要
半乳糖基甘露醇可通过β-半乳糖苷通透酶转运到大肠杆菌细胞中，并可被β-半乳糖苷酶迅速水解为甘露醇和半乳糖。当向缺乏磷酸烯醇式丙酮酸磷酸转移酶系统的酶I且乳糖系统组成型表达的突变菌株提供用(3)H标记甘露醇部分的半乳糖基甘露醇时，如果未诱导甘露醇的磷酸烯醇式丙酮酸磷酸转移酶系统的酶II复合物，则释放的甘露醇会保留在细胞内。据推测，即使无法进行磷酸化，酶II蛋白之一仍可催化甘露醇跨细胞膜的转运。
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