Panos C, Fagan G, Zarkadas C G
J Bacteriol. 1972 Oct;112(1):285-90. doi: 10.1128/jb.112.1.285-290.1972.
Major quantitative, but not qualitative, differences in the various species of proteins in purified membranes from Streptococcus pyogenes and its stabilized L-form have been demonstrated by acidic and alkaline disc gel electrophoresis with and without urea. The fact that no significant differences in the amino acid content or composition between these two membranes could be demonstrated emphasizes that these results are probably due to changes in the relative amounts of the various species of proteins in this subcellular component. The possibility of these protein changes in the L-form membrane being related to its inability to synthesize a rigid cell wall is discussed. Finally, phage-associated lysin, routinely used for removal of the group A streptococcal cell wall, does not appear to affect the protein profile or amino acid composition of the membrane either metabolically or nonmetabolically.
通过有无尿素存在下的酸性和碱性圆盘凝胶电泳，已证明化脓性链球菌及其稳定L型纯化膜中各种蛋白质在数量上存在主要差异，但在质量上无差异。这两种膜之间在氨基酸含量或组成上未显示出显著差异，这一事实强调这些结果可能是由于该亚细胞成分中各种蛋白质相对含量的变化所致。文中讨论了L型膜中这些蛋白质变化与其无法合成刚性细胞壁有关的可能性。最后，常用于去除A组链球菌细胞壁的噬菌体相关溶菌酶，似乎在代谢或非代谢方面均不影响膜的蛋白质谱或氨基酸组成。
