Rottem S, Razin S
J Bacteriol. 1967 Aug;94(2):359-64. doi: 10.1128/jb.94.2.359-364.1967.
Cell membranes of Mycoplasma were isolated either by osmotic lysis or by ultrasonic disruption of the organisms. The membranes were dissolved in phenol-acetic acid-water (2:1:0.5, w/v/v), and membrane proteins were separated electrophoretically in polyacrylamide gels containing 5 m urea and 35% (v/v) acetic acid. The electrophoretic patterns of membrane proteins were highly specific for the different Mycoplasma strains examined. The use of this method to prove the identity or dissimilarity of Mycoplasma strains is suggested.
支原体的细胞膜可通过对菌体进行渗透裂解或超声破碎来分离。将细胞膜溶解于苯酚 - 乙酸 - 水（2:1:0.5，w/v/v）中，然后在含有5M尿素和35%（v/v）乙酸的聚丙烯酰胺凝胶中对膜蛋白进行电泳分离。所检测的不同支原体菌株的膜蛋白电泳图谱具有高度特异性。建议使用该方法来证明支原体菌株的同一性或差异性。
