Gemski P, Alexeichik J A, Baron L S
J Virol. 1972 Oct;10(4):668-74. doi: 10.1128/JVI.10.4.668-674.1972.
The insensitivity of wild-type Shigella flexneri 2a to coliphage lambda is a consequence of its native genetic defect in the malA gene cluster. The "smooth" S. flexneri 2a lipopolysaccharide layer affects the efficient adsorption of lambda. Derivatives, capable of serving as functional hosts for lambda, were obtained by repairing the malA lesion, enabling the expression of the malB-lambdarcp region of S. flexneri. Introduction of a mutation into S. flexneri causing a "rough" lipopolysaccharide character resulted in more efficient adsorption of lambda. Such S. flexneri hosts can be stably lysogenized and upon induction yield gal(+)-transducing lysates. Lambda propagated on a malA(+) rough S. flexneri host was restricted by Escherichia coli K-12 and E. coli B, but not by E. coli C. This S. flexneri host did not restrict lambda grown on these E. coli strains.
野生型福氏志贺菌2a对λ噬菌体不敏感是其malA基因簇存在天然遗传缺陷的结果。“光滑”的福氏志贺菌2a脂多糖层影响λ噬菌体的有效吸附。通过修复malA损伤获得了能够作为λ噬菌体功能性宿主的衍生物，从而使福氏志贺菌的malB - λrcp区域得以表达。在福氏志贺菌中引入导致“粗糙”脂多糖特性的突变会使λ噬菌体的吸附更有效。这样的福氏志贺菌宿主可以稳定地溶源化，诱导后可产生半乳糖（+）转导裂解物。在malA（+）粗糙型福氏志贺菌宿主上繁殖的λ噬菌体受到大肠杆菌K - 12和大肠杆菌B的限制，但不受大肠杆菌C的限制。这种福氏志贺菌宿主不会限制在这些大肠杆菌菌株上生长的λ噬菌体。
