Ronen A, Raanan-Ashkenazi O
J Bacteriol. 1971 Jun;106(3):791-6. doi: 10.1128/jb.106.3.791-796.1971.
Escherichia coli B strains that have acquired the malB region from E. coli K-12 are able to utilize maltose and to adsorb phage lambda when grown at 30 C, but when grown at 40 C they do not absorb phage lambda and are devoid of amylomaltase activity. These Mal(ts) Lam(ts) cells can be mutated or transduced to become able to grow on maltose at 40 C, but they still have no detectable amylomaltase activity nor functional lambda receptors at that temperature. This Mal(40) phenotype is governed by a gene located near or at malA. It is suggested that the temperature sensitivity of both characters results from a defect in malT. However, transduction of malA from E. coli B to E. coli K-12 results in a wild-type phenotype, whereas E. coli B cells that have acquired malA from E. coli K-12 donors are still temperature sensitive for both amylomaltase and lambda-receptor production.
从大肠杆菌K-12获得malB区域的大肠杆菌B菌株，在30℃培养时能够利用麦芽糖并吸附噬菌体λ，但在40℃培养时它们不吸附噬菌体λ且缺乏淀粉麦芽糖酶活性。这些Mal(ts) Lam(ts)细胞可以通过突变或转导，从而能够在40℃的麦芽糖上生长，但在该温度下它们仍然没有可检测到的淀粉麦芽糖酶活性，也没有功能性的λ受体。这种Mal(40)表型由位于malA附近或malA处的一个基因控制。有人提出，这两个特性的温度敏感性是由malT缺陷导致的。然而，将malA从大肠杆菌B转导到大肠杆菌K-12会产生野生型表型，而从大肠杆菌K-12供体获得malA的大肠杆菌B细胞在淀粉麦芽糖酶和λ受体产生方面仍然对温度敏感。
