Püchel M, Müntz K, Parthier B, Aurich O, Bassüner R, Manteuffel R, Schmidt P
Eur J Biochem. 1979 May 15;96(2):321-9. doi: 10.1111/j.1432-1033.1979.tb13043.x.
In cotyledon cells of developing field beans the RNA content per cell does not change in the second half of developmental period 2, whereas globulin biosynthesis continues. The constant RNA content per cell results from an equilibrium between RNA synthesis and degradation. All types of RNA are synthesized until the end of globulin biosynthesis, but poly(A)-containing RNA was preferentially labelled during maximum globulin formation. During stage 2 of seed development of poly(A)-containing RNA fraction represents a discrete peak in the 12--18-S region on agarose gels and corresponds to the peak of poly(A)-containing RNA isolated from polysomes. alpha-Amanitin inhibits selectively the labelling of poly(A)-containing RNA and concomitantly globulin formation. Translation of total poly(A)-containing RNA, free and membrane-bound polysomes in a cell-free wheat germs demonstrates that the globulins are preferentially produced on membrane-bound polysomes and that poly(A)-containing RNA includes the mRNA for both vicilin and legumin.
在发育中的蚕豆子叶细胞中，在发育阶段2的后半期，每个细胞的RNA含量没有变化，而球蛋白的生物合成仍在继续。每个细胞中RNA含量恒定是RNA合成与降解之间平衡的结果。所有类型的RNA都持续合成直到球蛋白生物合成结束，但在球蛋白合成达到最大值时，含poly(A)的RNA被优先标记。在种子发育的第2阶段，含poly(A)的RNA组分在琼脂糖凝胶上的12 - 18S区域呈现一个离散峰，并且与从多核糖体分离的含poly(A)的RNA峰相对应。α-鹅膏蕈碱选择性地抑制含poly(A)的RNA的标记，并同时抑制球蛋白的形成。对无细胞小麦胚芽中游离和膜结合多核糖体上的总含poly(A)的RNA进行翻译表明，球蛋白优先在膜结合多核糖体上产生，并且含poly(A)的RNA包含豌豆球蛋白和豆球蛋白的mRNA。
