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狂犬病荧光抗体试验中免疫荧光的标准化与定量分析

Standardization and quantitation of immunofluorescence in the rabies fluorescent-antibody test.

作者信息

Trimarchi C V, Debbie J G

出版信息

Appl Microbiol. 1972 Oct;24(4):609-12. doi: 10.1128/am.24.4.609-612.1972.

Abstract

The standardization and quantitation of immunofluorescence with a Farrand microphotometer in the rabies fluorescent-antibody technique were determined. The system is useful in maintaining the quality of examinations for rabies in a public health laboratory since quantitation of reagent, equipment performance, and specificity of reaction can be evaluated. An analysis of the results demonstrates the high degree of reproducibility and the accuracy of the techniques described.

摘要

确定了在狂犬病荧光抗体技术中使用法朗德显微光度计进行免疫荧光标准化和定量的方法。该系统有助于维持公共卫生实验室狂犬病检测的质量,因为可以评估试剂的定量、设备性能和反应特异性。对结果的分析表明了所述技术具有高度的可重复性和准确性。

相似文献

2
[The diagnosis of rabies by immunofluorescence].
Arch Roum Pathol Exp Microbiol. 1970 Sep;29(3):523-32.

本文引用的文献

4
Fluorescent antibody method.荧光抗体法。
Folia Microbiol (Praha). 1962 May;7:155-61. doi: 10.1007/BF02928236.
6
An improved microfluorimeter for measuring brightness of fluorescent antibody reactions.
J Histochem Cytochem. 1967 Jan;15(1):38-45. doi: 10.1177/15.1.38.

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