肽链终止：蛋白质S对释放因子核糖体结合的影响。
Peptide chain termination: effect of protein S on ribosomal binding of release factors.
作者信息
Goldstein J L, Caskey C T
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):537-43. doi: 10.1073/pnas.67.2.537.
Abstract
THE PROTEIN FACTOR S, PREVIOUSLY SHOWN TO STIMULATE POLYPEPTIDE CHAIN TERMINATION IN BACTERIAL EXTRACTS, HAS TWO EFFECTS UPON THE COMPLEX FORMED BETWEEN RIBOSOMES, RELEASE FACTOR, AND TERMINATOR (TRINUCLEOTIDE) CODON: (1) in the absence of GTP or GDP, S stimulates formation of an [R.UAA.ribosome] intermediate, and (2) in the presence of GTP or GDP, S participates in dissociation of this intermediate. Factor S can stimulate fMet release from [fMet-tRNA(f).AUG.ribosome] intermediates in either the presence or absence of GTP or GDP. A model is proposed which relates the in vitro effects of S +/- GTP (or GDP) on fMet release to the effects of S +/- GTP (or GDP) on the binding and dissociation of R factor from ribosomes.
摘要
蛋白质因子S，先前已证明可刺激细菌提取物中的多肽链终止，它对核糖体、释放因子和终止子（三核苷酸）密码子之间形成的复合物有两种作用：（1）在没有GTP或GDP的情况下，S刺激[R.UAA.核糖体]中间体的形成，（2）在有GTP或GDP的情况下，S参与该中间体的解离。无论有无GTP或GDP，因子S都能刺激[fMet-tRNA(f).AUG.核糖体]中间体释放fMet。本文提出了一个模型，将S±GTP（或GDP）对fMet释放的体外作用与S±GTP（或GDP）对R因子与核糖体结合和解离的作用联系起来。
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