L-1-氯-4-苯基-3-甲苯-对磺酰胺基丁-2-酮（L-1-甲苯磺酰胺基-2-苯乙基氯甲基酮）对细菌多肽链起始复合物形成的抑制作用缺乏。
Lack of inhibition by L-1-chloro-4-phenyl-3-toluene-p-sulphonamidobutan-2-one (L-1-tosylamido-2-phenylethyl chloromethyl ketone) of the formation of bacterial polypeptide chain initiation complex.
作者信息
Jonák J, Clark B F
出版信息
Biochem J. 1973 Oct;136(2):433-6. doi: 10.1042/bj1360433.
Abstract
The chymotrypsin inhibitor l-1-chloro-4-phenyl-3-toluene-p-sulphonamidobutan-2-one does not inhibit the function of the initiation factor during the formation of the polypeptide chain initiation complex in vitro. Since the inhibitor has been shown previously to inhibit polypeptide chain elongation by reacting with elongation factor EF-Tu, the inhibitor can be used to investigate the initiation and elongation steps of protein biosynthesis separately.
摘要
糜蛋白酶抑制剂l-1-氯-4-苯基-3-甲苯-p-磺酰胺基丁烷-2-酮在体外多肽链起始复合物形成过程中不抑制起始因子的功能。由于该抑制剂先前已被证明可通过与延伸因子EF-Tu反应来抑制多肽链延伸，因此该抑制剂可用于分别研究蛋白质生物合成的起始步骤和延伸步骤。
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Lack of inhibition by L-1-chloro-4-phenyl-3-toluene-p-sulphonamidobutan-2-one (L-1-tosylamido-2-phenylethyl chloromethyl ketone) of the formation of bacterial polypeptide chain initiation complex.L-1-氯-4-苯基-3-甲苯-对磺酰胺基丁-2-酮（L-1-甲苯磺酰胺基-2-苯乙基氯甲基酮）对细菌多肽链起始复合物形成的抑制作用缺乏。
Biochem J. 1973 Oct;136(2):433-6. doi: 10.1042/bj1360433.
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Inactivation of protein-synthesizing T-factor by N-tosyl-L-phenylalanyl chloromethane.用N-对甲苯磺酰-L-苯丙氨酰氯甲烷使蛋白质合成T因子失活。
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