Lockwood A H, Chakraborty P R, Maitra U
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3122-6. doi: 10.1073/pnas.68.12.3122.
Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNA(f). This complex transfers both fMet-tRNA(f) and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNA(f) in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNA(f)] is an intermediate in the initiation of protein synthesis in Escherichia coli.
有证据表明,多肽链起始因子IF 2、GTP和fMet - tRNA(f)之间形成了一种复合物。在ApUpG和起始因子IF 1存在的情况下,这种复合物将fMet - tRNA(f)和GTP都转移到30S核糖体亚基上。形成的30S起始复合物与50S亚基反应形成70S起始复合物。这个70S复合物中的fMet - tRNA(f)与嘌呤霉素反应形成fMet - 嘌呤霉素。这些结果表明,[IF 2、GTP、fMet - tRNA(f)]是大肠杆菌蛋白质合成起始过程中的一个中间体。