Mulder C, Sharp P A, Delius H, Pettersson U
J Virol. 1974 Jul;14(1):68-77. doi: 10.1128/JVI.14.1.68-77.1974.
The products of complete digestion of duplex DNA of each of seven human adenoviruses with restriction endonuclease R. EcoRI ranged from two fragments for adenovirus 7 DNA (Ad7) to six fragments for Ad12 and Ad2 DNA. Viral serotypes from the same subgroups appeared to have related cleavage sites; Ad3 DNA and Ad7 (cl E46-LL) DNA were each cleaved into three fragments, and Ad7 (cl 19) DNA lacked one of the cleavage sites present in Ad3 and Ad7 (cl E46-LL) DNA. One of the cleavage sites in Ad2 DNA was deleted in the DNA' of adeno-SV40 hybrid virus Ad2(+)ND1, and three of the cleavage sites in Ad2 DNA were missing in Ad5 DNA. Thus, Ad2(+)ND1 DNA was cleaved into five and Ad5 DNA into three fragments. Each fragment represented a unique segment of viral DNA since each fragment was obtained in equimolar amounts and since the sum of the molecular weights of the fragments equaled the molecular weight of the homologous intact adenovirus DNA.
用限制性内切酶R. EcoRI完全消化七种人类腺病毒的双链DNA后，其产物从腺病毒7型DNA（Ad7）的两个片段到Ad12和Ad2 DNA的六个片段不等。来自同一亚组的病毒血清型似乎具有相关的切割位点；Ad3 DNA和Ad7（cl E46-LL）DNA均被切割成三个片段，而Ad7（cl 19）DNA缺少Ad3和Ad7（cl E46-LL）DNA中存在的一个切割位点。腺病毒-猴空泡病毒40杂交病毒Ad2(+)ND1的DNA中缺失了Ad2 DNA中的一个切割位点，Ad5 DNA中缺失了Ad2 DNA中的三个切割位点。因此，Ad2(+)ND1 DNA被切割成五个片段，Ad5 DNA被切割成三个片段。每个片段代表病毒DNA的一个独特区段，因为每个片段都是以等摩尔量获得的，并且片段的分子量总和等于同源完整腺病毒DNA的分子量。
