大肠杆菌K-12中氨酰基转移核糖核酸合成酶调节突变体的分离与鉴定。
Isolation and characterization of a regulatory mutant of an aminoacyl-transfer ribonucleic acid synthetase in Escherichia coli K-12.
作者信息
Clarke S J, Low B, Konigsberg W
出版信息
J Bacteriol. 1973 Mar;113(3):1096-103. doi: 10.1128/jb.113.3.1096-1103.1973.
Abstract
From Escherichia coli strain K28, which is temperature sensitive for growth because of a mutation in its seryl-transfer ribonucleic acid (tRNA) synthetase gene (serS), temperature-resistant mutants were selected which were found to have a fivefold higher level of seryl-tRNA synthetase than the parent strain. The "high-level" character was found to be genetically stable and is due to a mutation in a locus denoted serO. This locus was found to be very closely linked to serS on the genetic map, and the relative gene order was concluded to be serS-serO-serC. In a serO(-) strain, the normal dependence of seryl-tRNA synthetase (SerRS) activity on changes of exogenous serine concentration was not observed. In a stable heterozygous merodiploid, the serO(-) mutation is still expressed, i.e., it is cis dominant. These results strongly suggest that serO is an operator site involved in the control of the serS gene.
摘要
从大肠杆菌K28菌株中筛选出温度抗性突变体，该菌株因其丝氨酰 - 转移核糖核酸（tRNA）合成酶基因（serS）发生突变而对生长温度敏感，发现这些突变体的丝氨酰 - tRNA合成酶水平比亲本菌株高五倍。“高水平”特征在遗传上是稳定的，这是由于一个名为serO的位点发生了突变。发现该位点在遗传图谱上与serS紧密连锁，相对基因顺序为serS - serO - serC。在serO（ - ）菌株中，未观察到丝氨酰 - tRNA合成酶（SerRS）活性对外源丝氨酸浓度变化的正常依赖性。在稳定的杂合部分二倍体中，serO（ - ）突变仍然表达，即它是顺式显性的。这些结果强烈表明serO是一个参与serS基因控制的操纵位点。
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