Russell R R, Pittard A J
J Bacteriol. 1971 Nov;108(2):790-8. doi: 10.1128/jb.108.2.790-798.1971.
Members of a collection of mutants of Escherichia coli unable to form colonies on nutrient agar at 42 C have been characterized on the basis of their growth response to a shift from 32 to 42 C in liquid medium. Forty-four mutants, which show an abrupt, nonlethal cessation of growth when moved to the restrictive temperature, have been characterized with respect to the effect of the mutation responsible for temperature sensitivity on deoxyribonucleic acid, ribonucleic acid, and protein synthesis. In 12 mutants, the mutation causing temperature sensitivity of growth primarily affects protein synthesis, in each case through an altered aminoacyl-transfer ribonucleic acid synthetase. Mutants with temperature-sensitive glutamyl-, phenylalanyl-, and valyl-transfer ribonucleic acid synthetases have been obtained, and the genes specifying these enzymes have been mapped by conjugation and transduction. Another mutant has been shown to possess a temperature-sensitive tryptophanyl-transfer ribonucleic acid synthetase, but this is not responsible for inability to grow at 42 C on media containing tryptophan.
一组无法在42℃的营养琼脂上形成菌落的大肠杆菌突变体，已根据它们在液体培养基中从32℃转移到42℃时的生长反应进行了表征。44个突变体在转移到限制温度时表现出突然的、非致死性的生长停止，已就导致温度敏感性的突变对脱氧核糖核酸、核糖核酸和蛋白质合成的影响进行了表征。在12个突变体中，导致生长温度敏感性的突变主要影响蛋白质合成，每种情况下都是通过改变氨酰基转移核糖核酸合成酶来实现的。已获得具有温度敏感性谷氨酰胺、苯丙氨酸和缬氨酸转移核糖核酸合成酶的突变体，并通过接合和转导对指定这些酶的基因进行了定位。另一个突变体已被证明具有温度敏感性色氨酸转移核糖核酸合成酶，但这并不是在含有色氨酸的培养基上无法在42℃生长的原因。
