脂多糖对源自骨髓的小鼠淋巴细胞的促有丝分裂作用。脂多糖A作为该分子的促有丝分裂部分。
The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule.
作者信息
Andersson J, Melchers F, Galanos C, Lüderitz O
出版信息
J Exp Med. 1973 Apr 1;137(4):943-53. doi: 10.1084/jem.137.4.943.
Abstract
Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action.
摘要
从大肠杆菌和沙门氏菌的不同突变菌株中分离出的具有不同结构的脂多糖，以及这些脂多糖的化学降解产物，已被用于研究脂多糖分子的哪一部分对源自骨髓的小鼠淋巴细胞发挥促有丝分裂作用。在由脂质A、核心多糖和O-多糖抗原组成的脂多糖结构中，发现脂质A是具有促有丝分裂作用的部分。由带有酯键和酰胺键连接脂肪酸的磷酸化葡糖胺二糖单元组成的脂质A的促有丝分裂作用，在碱处理后消失，碱处理会去除酯键连接的脂肪酸。有人讨论了将脂多糖的脂质A部分插入源自骨髓的淋巴细胞质膜的脂质双层中是最初的促有丝分裂作用。
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本文引用的文献
1
THE NATURAL-SELECTION THEORY OF ANTIBODY FORMATION.抗体形成的自然选择理论。
Proc Natl Acad Sci U S A. 1955 Nov 15;41(11):849-57. doi: 10.1073/pnas.41.11.849.
2
O-PHOSPHORYLETHANOLAMINE: A COMPONENT OF LIPOPOLYSACCHARIDE IN CERTAIN GRAM-NEGATIVE BACTERIA.O-磷酸乙醇胺：某些革兰氏阴性菌中脂多糖的一种成分。
Biochemistry. 1964 Oct;3:1571-4. doi: 10.1021/bi00898a031.
3
4
A new method for the extraction of R lipopolysaccharides.一种提取R脂多糖的新方法。
Eur J Biochem. 1969 Jun;9(2):245-9. doi: 10.1111/j.1432-1033.1969.tb00601.x.
5
Effect of the number of haptens coupled to each erythrocyte on haemolytic plaque formation.每个红细胞上结合的半抗原数量对溶血空斑形成的影响。
Immunology. 1969 Mar;16(3):399-407.
6
Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.抗三硝基苯基（TNP）空斑试验。Balb/c小鼠对可溶性和颗粒性免疫原的初次反应。
Proc Soc Exp Biol Med. 1969 Nov;132(2):575-81. doi: 10.3181/00379727-132-34264.
7
Nature and linkages of the fatty acids present in the lipid-A component of Salmonella lipopolysaccharides.沙门氏菌脂多糖脂质A成分中存在的脂肪酸的性质和联系。
Eur J Biochem. 1972 Jul 13;28(2):166-73. doi: 10.1111/j.1432-1033.1972.tb01899.x.
8
Role of lipids in the biosynthesis of the bacterial cell envelope.脂质在细菌细胞壁生物合成中的作用。
Bacteriol Rev. 1971 Mar;35(1):14-38. doi: 10.1128/br.35.1.14-38.1971.
9
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Transplant Rev. 1972;11:131-77. doi: 10.1111/j.1600-065x.1972.tb00048.x.
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