Tanwar Himanshi, Shukla Shruti, Ganju Lilly
Department of Advanced Oral Sciences and Therapeutics, University of Maryland, Baltimore, 21201, India.
Index Group of Institutions, Malwanchal University, Index City, Indore, 452016, India.
Inflammopharmacology. 2025 Jul 12. doi: 10.1007/s10787-025-01825-8.
The new generation vaccines containing purified antigens are safer to use but generally induce weaker immune responses. Therefore, purified subunit vaccines require the addition of an exogenous adjuvant to enhance the immune response to the antigens following immunization. In the present study, a molecule has been isolated and characterized from 70% ethanolic extract of the medicinal plant Hippophaerhamnoides L. (Elaeagnaceae), commonly known as seabuckthorn (SBT). In this study, we have fractionated SBT leaf extract using solvents to identify the bioactive compound(s) and evaluated its adjuvant efficacy with weak antigen ovalbumin in balb/c mice. The identified bioactive compound was found to have a hydrocarbon moiety and was identified as alkane (named SSFa1), which in combination with ovalbumin boost humoral response in terms of high antibody titers, cell-mediated immune response such as T cell activation marker (CD27), and increased nitric oxide and proinflammatory cytokines (TNF-a and IL-6) production by peritoneal macrophages.
新一代含有纯化抗原的疫苗使用起来更安全，但通常诱导的免疫反应较弱。因此，纯化亚单位疫苗需要添加外源性佐剂以增强免疫接种后对抗原的免疫反应。在本研究中，已从药用植物沙棘（胡颓子科）70%乙醇提取物中分离并鉴定出一种分子，沙棘俗称醋柳（SBT）。在本研究中，我们使用溶剂对沙棘叶提取物进行分级分离以鉴定生物活性化合物，并在Balb/c小鼠中评估其与弱抗原卵清蛋白的佐剂功效。所鉴定的生物活性化合物含有一个烃基部分，被鉴定为烷烃（命名为SSFa1），它与卵清蛋白结合可提高抗体滴度，增强体液免疫反应，促进细胞介导的免疫反应，如T细胞活化标志物（CD27），并增加腹膜巨噬细胞产生一氧化氮和促炎细胞因子（TNF-α和IL-6）。
