Pasanen V J, Mäkelä O
Immunology. 1969 Mar;16(3):399-407.
We varied the density of a hapten coupled to sheep erythrocytes (SRC), and used different hapten—erythrocyte conjugates to study single antibody forming cells from rabbits. Both direct and indirect (developed by anti-IgG) plaques were seen. Hapten density influenced both plaque number and size. With aliquots of the same lymphoid cell suspension heavily coupled SRC yielded more direct and more indirect plaques than lightly coupled SRC. This tendency decreased with continuing immunization. Free hapten inhibited the formation of plaques more easily in lightly than in heavily coupled erythrocytes. Direct plaques were more dependent on a dense epitope coat than indirect ones. Our data indicated, that cells producing IgG antibody could produce direct plaques. To explain our data we suggest that the product of some anti-hapten producing cells needs the collaboration of several combining sites to bind efficiently onto the hapten—SRC. They are dependent on a dense coat. The product of other cells has a higher affinity per site and binds efficiently onto the lightly coupled SRC.
我们改变了与绵羊红细胞（SRC）偶联的半抗原的密度，并使用不同的半抗原 - 红细胞缀合物来研究来自兔子的单个抗体形成细胞。观察到了直接斑和间接（由抗IgG产生）斑。半抗原密度影响斑的数量和大小。用相同淋巴样细胞悬液的等分试样，与轻度偶联的SRC相比，重度偶联的SRC产生更多的直接斑和更多的间接斑。随着持续免疫，这种趋势减弱。游离半抗原在轻度偶联的红细胞中比在重度偶联的红细胞中更容易抑制斑的形成。直接斑比间接斑更依赖于密集的表位包被。我们的数据表明，产生IgG抗体的细胞可以产生直接斑。为了解释我们的数据，我们提出一些产生抗半抗原细胞的产物需要几个结合位点的协作才能有效地结合到半抗原 - SRC上。它们依赖于密集的包被。其他细胞的产物每个位点具有更高的亲和力，并有效地结合到轻度偶联的SRC上。
