Hearn W R, Williams R H, Burgus R C, Williams R P
Appl Microbiol. 1972 Oct;24(4):591-5. doi: 10.1128/am.24.4.591-595.1972.
Countercurrent distribution is capable of resolving mixtures of closely related prodigiosene pigments. Syntrophic pigment produced by several pairs of Serratia marcescens color mutants was identified as prodigiosin (2-methyl-3-amyl-6-methoxyprodigiosene) by countercurrent distribution, soda lime pyrolysis, and other techniques. The metabolic block of mutant strain H-462, derived from parent strain HY, was located between the blocks of mutant strains OF and WF, both derived from parent strain Nima.
逆流分配能够分离密切相关的灵菌红素色素混合物。通过逆流分配、苏打石灰热解及其他技术,由几对粘质沙雷氏菌颜色突变体产生的共营色素被鉴定为灵菌红素(2-甲基-3-戊基-6-甲氧基灵菌红素)。源自亲本菌株HY的突变菌株H-462的代谢阻断位于源自亲本菌株尼玛的突变菌株OF和WF的阻断之间。