Tadano Y, Yamada K
Histochemistry. 1979 Apr 3;60(2):125-33. doi: 10.1007/BF00495748.
In the intercellular matrix of the granulosa layer of the mouse ovarian follicles, ultrastructural features of acidic complex carbohydrates have been studied by means of dialyzed iron (DI) staining in combination with procedures of digestion with Streptomyces and testicular hyaluronidases. In the intercellular matrix, DI reactive structures containing acidic complex carbohydrates consist of layers of a variable thickness coating the plasma membrane of the granulosa cells and reticular elements distributed in the spaces between the cells. The latter exists in two appearances; one is clumped masses of irregular shapes and different sizes, whereas the other being filamentous figures radiating from the masses. The effects of digestion with Streptomyces and testicular hyaluronidases upon the DI staining of the tissues indicate that the DI reactive structures in the intercellular matrix contain at least three types of acidic complex carbohydrates; hyaluronic acid, isomeric chondroitin sulfates and other acidic glycosaminoglycans. The histophysiological activities played by these particular complex carbohydrates have been briefly discussed.
在小鼠卵巢卵泡颗粒层的细胞间基质中，通过透析铁（DI）染色结合链霉菌和睾丸透明质酸酶消化程序，研究了酸性复合碳水化合物的超微结构特征。在细胞间基质中，含有酸性复合碳水化合物的DI反应性结构由覆盖颗粒细胞膜的可变厚度层和分布在细胞间空间的网状元素组成。后者有两种外观；一种是不规则形状和不同大小的团块，另一种是从团块放射出的丝状图形。链霉菌和睾丸透明质酸酶消化对组织DI染色的影响表明，细胞间基质中的DI反应性结构至少含有三种酸性复合碳水化合物；透明质酸、异构硫酸软骨素和其他酸性糖胺聚糖。已简要讨论了这些特定复合碳水化合物所发挥的组织生理活性。
