Tadano Y, Yamada K
Histochemistry. 1978 Sep 15;57(3):203-15. doi: 10.1007/BF00492080.
In the ovarian follicles of adult mice, complex carbohydrate-containing structures have been studied by means of light microscopic histochemical methods. In the ovarian follicles, the zona pellucida of oocytes, follicular fluid and intercellular matrix of the granulosa layer are found to exhibit positive reactions for complex carbohydrates with 1,2-glycol and acidic groups and alpha-D-mannosyl and alpha-D-glucosyl residues. The present histochemical analyses have revealed that the complex carbohydrates common to the three types of the structures are hyaluronic acid, sulfated glycosaminoglycans other than isomeric chondroitin sulfates and neutral glycoproteins and that sialic acid is a particular moiety of the zona pellucida, whereas isomeric chondroitin sulfates being that of the follicular fluid and intercellular matrix of the granulosa layer. The histophysiological activities of the carbohydrate-containing structures have been discussed with special reference to their histochemical properties determined in the present study.
在成年小鼠的卵巢卵泡中，已通过光学显微镜组织化学方法对含复合碳水化合物的结构进行了研究。在卵巢卵泡中，发现卵母细胞的透明带、卵泡液和颗粒层的细胞间基质对具有1,2 -二醇和酸性基团以及α - D -甘露糖基和α - D -葡萄糖基残基的复合碳水化合物呈现阳性反应。目前的组织化学分析表明，这三种结构共有的复合碳水化合物是透明质酸、除异构硫酸软骨素外的硫酸化糖胺聚糖和中性糖蛋白，并且唾液酸是透明带的特定组成部分，而异构硫酸软骨素是卵泡液和颗粒层细胞间基质的组成部分。已结合本研究确定的组织化学特性，对含碳水化合物结构的组织生理活性进行了讨论。
