通过溶菌酶攻击可检测到的大肠杆菌中的渗透压。
Osmotic pressure in Escherichia coli as rendered detectable by lysozyme attack.
作者信息
Scheie P
出版信息
J Bacteriol. 1973 May;114(2):549-55. doi: 10.1128/jb.114.2.549-555.1973.
Abstract
The enhanced susceptibility of plasmolyzed Escherichia coli to lysozyme attack was used to estimate the internal osmotic pressure of these cells under various conditions. Differences were detected between strains, culture media, stages in the growth cycle, and the osmotically active material used to produce plasmolysis. Lysozyme also was found to attack unplasmolyzed cells at 0 C and between 50 and 70 C.
摘要
利用质壁分离的大肠杆菌对溶菌酶攻击的增强敏感性来估计这些细胞在各种条件下的内部渗透压。在菌株、培养基、生长周期阶段以及用于产生质壁分离的渗透活性物质之间检测到差异。还发现溶菌酶在0℃以及50至70℃之间会攻击未发生质壁分离的细胞。
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