Scheie P O
J Bacteriol. 1969 May;98(2):335-40. doi: 10.1128/jb.98.2.335-340.1969.
Escherichia coli B/r cells were plasmolyzed in sucrose solutions and observed under phase contrast. The prevalence of plasmolysis under various conditions was noted, and the degree of plasmolysis was categorized as slight, extensive, or severe. The presence of ions reduced the prevalence of plasmolysis. Survival curves showed that extensive plasmolysis was not lethal to colony-forming ability.
将大肠杆菌B/r细胞在蔗糖溶液中进行质壁分离,并在相差显微镜下观察。记录了不同条件下质壁分离的发生率,并将质壁分离的程度分为轻微、广泛或严重。离子的存在降低了质壁分离的发生率。存活曲线表明,广泛的质壁分离对菌落形成能力并不致命。