Russell R R
J Bacteriol. 1972 Nov;112(2):661-5. doi: 10.1128/jb.112.2.661-665.1972.
A collection of temperature-sensitive mutants of Escherichia coli K-12 was examined for ability to grow at the restrictive temperature when the osmotic pressure of the medium was increased. Five of the fourteen mutants were found to be osmotic remedial. Four strains containing temperature-sensitive, osmotic-remedial mutations affecting aminoacyl-transfer ribonucleic acid synthetases were found to have altered permeability characteristics which may be attributable to changes in the lipopolysaccharide layer of the cell envelope at restrictive temperatures.
检测了一组大肠杆菌K-12的温度敏感突变体在培养基渗透压升高时于限制温度下生长的能力。在14个突变体中,有5个被发现具有渗透压修复能力。发现4个含有影响氨酰转移核糖核酸合成酶的温度敏感、渗透压修复突变的菌株具有改变的通透性特征,这可能归因于在限制温度下细胞包膜脂多糖层的变化。