Dudun Andrei A, Akoulina Elizaveta A, Zhuikov Vsevolod A, Makhina Tatiana K, Voinova Vera V, Belishev Nikita V, Khaydapova Dolgor D, Shaitan Konstantin V, Bonartseva Garina A, Bonartsev Anton P
Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave, 33, Bld. 2, 119071 Moscow, Russia.
Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.
Polymers (Basel). 2021 Dec 30;14(1):131. doi: 10.3390/polym14010131.
This study investigated the effect of various cultivation conditions (sucrose/phosphate concentrations, aeration level) on alginate biosynthesis using the bacterial producing strain 12 by the full factorial design (FFD) method and physicochemical properties (e.g., rheological properties) of the produced bacterial alginate. We demonstrated experimentally the applicability of bacterial alginate for tissue engineering (the cytotoxicity testing using mesenchymal stem cells (MSCs)). The isolated synthesis of high molecular weight (M) capsular alginate with a high level of acetylation (25%) was achieved by FFD method under a low sucrose concentration, an increased phosphate concentration, and a high aeration level. Testing the viscoelastic properties and cytotoxicity showed that bacterial alginate with a maximal M (574 kDa) formed the densest hydrogels (which demonstrated relatively low cytotoxicity for MSCs in contrast to bacterial alginate with low M). The obtained data have shown promising prospects in controlled biosynthesis of bacterial alginate with different physicochemical characteristics for various biomedical applications including tissue engineering.
本研究采用全因子设计（FFD）方法，研究了不同培养条件（蔗糖/磷酸盐浓度、通气水平）对产藻酸盐细菌菌株12藻酸盐生物合成的影响，以及所产细菌藻酸盐的物理化学性质（如流变学性质）。我们通过实验证明了细菌藻酸盐在组织工程中的适用性（使用间充质干细胞（MSCs）进行细胞毒性测试）。通过FFD方法，在低蔗糖浓度、增加的磷酸盐浓度和高通气水平下，实现了高分子量（M）、高乙酰化水平（25%）的荚膜藻酸盐的分离合成。对粘弹性性质和细胞毒性的测试表明，具有最大M值（574 kDa）的细菌藻酸盐形成了最致密的水凝胶（与低M值的细菌藻酸盐相比，对MSCs表现出相对较低的细胞毒性）。所获得的数据表明，在可控生物合成具有不同物理化学特性的细菌藻酸盐以用于包括组织工程在内的各种生物医学应用方面，具有广阔的前景。
