大肠杆菌素B：作用模式及肠螯合素对其的抑制作用
Colicin B: mode of action and inhibition by enterochelin.
作者信息
Guterman S K
出版信息
J Bacteriol. 1973 Jun;114(3):1217-24. doi: 10.1128/jb.114.3.1217-1224.1973.
Abstract
Adsorption of colicin B to a sensitive strain of Escherichia coli results in rapid cessation of deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Some classes of mutants insensitive to colicin B hyperexcrete a colicin inhibitor into their growth medium. This inhibitor functions by preventing adsorption of colicin B and does not rescue cells to which colicin has already adsorbed. The inhibitor is insensitive to nucleases, proteolytic enzymes, and lysozyme and is not extracted into organic solvents. The inhibitory material has a low molecular weight, which rules out identification as lipopolysaccharide, although purified lipopolysaccharide has some inhibitory activity. Evidence is presented that the inhibitor is enterochelin, an iron chelator which is the cyclic trimer of 2,3-dihydroxybenzoylserine. Enterochelin does not inhibit colicin M, a colicin that is produced by many strains colicinogenic for colicin B.
摘要
大肠杆菌素B吸附到敏感的大肠杆菌菌株上会导致脱氧核糖核酸、核糖核酸和蛋白质合成迅速停止。一些对大肠杆菌素B不敏感的突变体类会向其生长培养基中超量分泌一种大肠杆菌素抑制剂。这种抑制剂通过阻止大肠杆菌素B的吸附起作用，并且不能拯救已经吸附了大肠杆菌素的细胞。该抑制剂对核酸酶、蛋白水解酶和溶菌酶不敏感，也不能被有机溶剂萃取。这种抑制物质的分子量较低，这排除了它被鉴定为脂多糖的可能性，尽管纯化的脂多糖具有一些抑制活性。有证据表明该抑制剂是肠螯合素，一种铁螯合剂，它是2,3 - 二羟基苯甲酰丝氨酸的环状三聚体。肠螯合素不抑制大肠杆菌素M，大肠杆菌素M是由许多对大肠杆菌素B产大肠杆菌素的菌株产生的一种大肠杆菌素。
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