大肠杆菌K-12的B组大肠菌素抗性突变体的特性：大肠菌素抗性与肠螯合素的作用
Characterization of group B colicin-resistant mutants of Escherichia coli K-12: colicin resistance and the role of enterochelin.
作者信息
Pugsley A P, Reeves P
出版信息
J Bacteriol. 1976 Jul;127(1):218-28. doi: 10.1128/jb.127.1.218-228.1976.
Abstract
Nine classes of group B colicin-resistant mutants were examined to study the role of enterochelin in colicin resistance. Four of the mutants studied (cbt, exbC, exbB, and tonB) hypersecreted enterochelin. Enterochelin hypersecretion was apparently responsible for resistance of the exbC mutant to colicins G and H and for resistance of the exbB mutant to colicins G, H, Ia, Ib, S1, and V. All four mutants scored as colicin B tolerant, even in the absence of enterochelin synthesis. The mutants produced substantially increased amounts of two high-molecular-weight outer membrane polypeptides when grown under limiting iron conditions. The presence of these polypeptides was correlated with increased colicin B-neutralizing activity in the outer membrane preparations.
摘要
为研究肠螯合素在大肠杆菌素抗性中的作用，对九类B组大肠杆菌素抗性突变体进行了检测。所研究的四个突变体（cbt、exbC、exbB和tonB）过度分泌肠螯合素。肠螯合素的过度分泌显然是exbC突变体对大肠杆菌素G和H产生抗性以及exbB突变体对大肠杆菌素G、H、Ia、Ib、S1和V产生抗性的原因。即使在没有肠螯合素合成的情况下，所有这四个突变体对大肠杆菌素B均表现出耐受。当在铁限制条件下生长时，这些突变体产生的两种高分子量外膜多肽的量大幅增加。这些多肽的存在与外膜制剂中大肠杆菌素B中和活性的增加相关。
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