van Kampen C L, Gibbons D F
J Biomed Mater Res. 1979 Jul;13(4):517-41. doi: 10.1002/jbm.820130402.
A series of poly(alpha-amino acid)s with controlled chemical variations were investigated in order to assess the effect of different chemical moieties upon arterial thrombosis. The gross implant surface properties ranged from hydrophobic to hydrophilic ionic and nonionic. The materials were tested by implantation within canine femoral and carotid arteries. Results were compared with the response to the polyurethane Biomer. The changes in implant surface chemistry elicited a range of response that varied from intense thrombosis and rapid vessel occlusion to minimal thrombosis and endothelialization. The results showed that no simple relationship exists between a gross surface property, such as hydrophobicity, and the degree of thrombosis resistance. Some hydrophobic and hydrophilic materials were found to have good thrombosis resistance, while others were found to have poor thrombosis resistance. Leukocytes were shown to play an important role in both initial thrombosis and endothelialization. The major difference between materials that progressed to rapid vessel occlusion and materials that remained patent was the degree of direct leukocyte adherence and spreading on the implant surface prior to extensive platelet aggregation (less than 30 min). It was consistent for both hydrophobic and hydrophilic materials that the lack of direct leukocyte adherence to the implant surface was associated with intense thrombosis and rapid vessel occlusion. Conversely, the presence of numerous leukocytes directly adherent to either hydrophobic or hydrophilic surfaces appeared to have a moderating effect upon thrombosis and vessels with these implants remained patent. In instances when thrombosis was nonocclusive, the surfaces of the thrombi became endothelialized, primarily through the transformation of mononuclear leukocytes into endothelial cells. This article includes a hypothetical model representing the sequence of events and alternative pathways occurring at the blood-material interface, with special attention given to the involvement of leukocytes in arterial thrombosis.
研究了一系列具有可控化学变化的聚（α-氨基酸），以评估不同化学基团对动脉血栓形成的影响。植入物的总体表面性质范围从疏水性到亲水性离子和非离子性。通过将材料植入犬股动脉和颈动脉来进行测试。将结果与对聚氨酯Biomer的反应进行比较。植入物表面化学的变化引发了一系列反应，从强烈的血栓形成和快速的血管闭塞到最小的血栓形成和内皮化。结果表明，诸如疏水性等总体表面性质与抗血栓形成程度之间不存在简单的关系。发现一些疏水性和亲水性材料具有良好的抗血栓形成能力，而另一些则具有较差的抗血栓形成能力。白细胞在初始血栓形成和内皮化过程中均发挥重要作用。进展为快速血管闭塞的材料与保持通畅的材料之间的主要区别在于，在广泛的血小板聚集之前（少于30分钟），白细胞在植入物表面直接粘附和铺展的程度。对于疏水性和亲水性材料而言，一致的是白细胞缺乏直接粘附于植入物表面与强烈的血栓形成和快速的血管闭塞有关。相反，大量白细胞直接粘附于疏水性或亲水性表面似乎对血栓形成具有调节作用，并且带有这些植入物的血管保持通畅。在血栓形成非闭塞的情况下，血栓表面主要通过单核白细胞转化为内皮细胞而发生内皮化。本文包括一个假设模型，该模型代表了在血液-材料界面发生的一系列事件和替代途径，并特别关注白细胞在动脉血栓形成中的作用。
