Vilardell J, Chartrand P, Singer R H, Warner J R
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
RNA. 2000 Dec;6(12):1773-80. doi: 10.1017/s135583820000145x.
The transcript of the Saccharomyces cerevisiae gene, RPL30, is subject to regulated splicing and regulated translation, due to a structure that interacts with its own product, ribosomal protein L30. We have followed the fate of the regulated RPL30 transcripts in vivo. Initially, these transcripts abortively enter the splicing pathway, forming an unusually stable association with U1 snRNP. A large proportion of the unspliced molecules, however, are found in the cytoplasm. Most of these are still bound by L30, as only a small fraction are engaged in translation. Eventually, the unspliced RPL30 transcripts escape the grasp of L30, associate with ribosomes, and fall prey to nonsense mediated decay.
酿酒酵母基因RPL30的转录本会受到可变剪接和翻译调控,这是由于其结构能与其自身产物核糖体蛋白L30相互作用。我们在体内追踪了受调控的RPL30转录本的命运。最初,这些转录本异常地进入剪接途径,与U1 snRNP形成异常稳定的结合。然而,大部分未剪接的分子存在于细胞质中。其中大多数仍与L30结合,因为只有一小部分参与翻译。最终,未剪接的RPL30转录本逃脱L30的束缚,与核糖体结合,并成为无义介导衰变的牺牲品。