Ryan J M
J Cell Physiol. 1979 Apr;99(1):67-77. doi: 10.1002/jcp.1040990109.
We have examined the kinetics of chick cell population aging in vitro using the percentage of labeled nuclei, the number of colonies formed from a low density inoculum and the number of cells/colony to monitor culture age. The results from these studies showed a gradual age-associated decline in each of the parameters which was first detected early in the culture lifespan and well in advance of changes in total cell number at confluency. Our results also indicated that each of the above parameters, in addition to the calendar time cells had been in culture, could be used to estimate the percentage of lifespan completed by the culture. A comparison of the methods used to estimate the remaining culture lifespan indicated that the percentage of labeled nuclei was the most accurate in describing cell age.
我们利用标记细胞核的百分比、低密度接种物形成的集落数量以及每个集落中的细胞数量来监测培养物的老化程度，研究了鸡细胞群体在体外老化的动力学。这些研究结果表明，在培养寿命早期就首次检测到每个参数都随着年龄的增长而逐渐下降，并且在汇合时总细胞数发生变化之前就已经出现这种情况。我们的结果还表明，除了细胞在培养中的日历时间外，上述每个参数都可用于估计培养物完成的寿命百分比。对用于估计剩余培养寿命的方法进行比较表明，标记细胞核的百分比在描述细胞年龄方面最为准确。
