Ryan J M
In Vitro. 1979 Nov;15(11):895-9. doi: 10.1007/BF02618046.
The effect of Eagle's minimal essential medium, containing different fetal bovine serum (FBS) concentrations, on the proliferation and replicative life span of cultured chick cells has been studied. Our results showed that the rate of chick cell proliferation and the cell density at stationary phase increased as a function of serum concentration between 5 and 30% FBS. The replicative life span of cultured chick cells was dependent on the FBS concentration between 5 and 20% in a medium volume of 0.20 ml/cm2. The maximum replicative life span of chick cells was obtained by serially propagating cells in a medium volume of 0.20 ml/cm2 containing 20 or 30% FBS, or, alternatively, in 0.53 ml/cm2 containing 10, 20 or 30% FBS. Cells grown in medium containing 5% serum had a calendar life span of 35 days, whereas cells propagated in medium containing higher serum concentrations had a calendar life span of 50 days. These results reenforce the concept that, although the kinetics of cell population aging can be affected by the culture medium composition, the aging of cells in culture is controlled by alterations within the cell.
研究了含有不同浓度胎牛血清（FBS）的伊格尔氏最低必需培养基对培养的鸡细胞增殖和复制寿命的影响。我们的结果表明，在5%至30% FBS的血清浓度范围内，鸡细胞的增殖速率和静止期的细胞密度随血清浓度的增加而增加。在0.20 ml/cm²的培养基体积中，培养的鸡细胞的复制寿命取决于5%至20%的FBS浓度。通过在含有20%或30% FBS的0.20 ml/cm²培养基体积中连续传代细胞，或者在含有10%、20%或30% FBS的0.53 ml/cm²培养基体积中连续传代细胞，可以获得鸡细胞的最大复制寿命。在含有5%血清的培养基中生长的细胞日历寿命为35天，而在含有较高血清浓度的培养基中传代的细胞日历寿命为50天。这些结果强化了这样一个概念，即尽管细胞群体衰老的动力学可以受到培养基组成的影响，但培养细胞的衰老由细胞内的变化控制。
